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2.12 Lipidomics analysis The cell samples were harvested directly with a cell scraper after 24 h of treatment with precooled solution (methanol:acetonitrile:water = 2:2:1), followed by transfer into a 1.5 mL centrifuge tube

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One of the few transplantation studies that quantitatively evaluated tN myelination showed that fetal rat spinal cord NPC grafts after C5 hemisection displayed myelination by host OLs in around 24% of tN axons emerging from the graft quantified 814 mm caudal to the lesion site (C8), with a G-ratio equal to that of axons in the intact spinal cord (Hunt et al., 2017)